Optimisation of Widefield Fluorescence Fret System for Studying Separate Molecule Interactions
نویسندگان
چکیده
منابع مشابه
Studying DNA looping by single-molecule FRET.
Bending of double-stranded DNA (dsDNA) is associated with many important biological processes such as DNA-protein recognition and DNA packaging into nucleosomes. Thermodynamics of dsDNA bending has been studied by a method called cyclization which relies on DNA ligase to covalently join short sticky ends of a dsDNA. However, ligation efficiency can be affected by many factors that are not relat...
متن کاملNanovesicle trapping for studying weak protein interactions by single-molecule FRET.
Protein-protein interactions are fundamental biological processes. While strong protein interactions are amenable to many characterization techniques including crystallography, weak protein interactions are challenging to study because of their dynamic nature. Single-molecule fluorescence resonance energy transfer (smFRET) can monitor dynamic protein interactions in real time, but are generally...
متن کاملStudying DNA translocation in nanocapillaries using single molecule fluorescence.
We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axi...
متن کاملSingle-molecule switchable FRET
Two-photon microscopy offers unique advantages for excitation of channelrhodopsin-2 (ChR2)-expressing neurons, but previous attempts had limitations in terms of the axial and temporal resolution. Emiliani and colleagues now use a scanless approach that combines generalized phase contrast and temporal focusing to shape two-photon excitation patterns and trigger single action potentials or trains...
متن کاملLabeling proteins for single-molecule FRET.
Single-molecule (sm) fluorescence detection is a powerful method for studying biological events without time and population averaging. Förster (fluorescence) resonance energy transfer (FRET) is a spectroscopic technique for measuring distances in the 30-80 Å range in which excitation energy of a donor molecule is transferred to an acceptor via interaction between two induced dipoles. A variant ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.
سال: 2018
ISSN: 1407-009X
DOI: 10.2478/prolas-2018-0065